Histology & Bioimaging Core
The ACNC Histology and Bioimaging Core assists ACNC investigators with all aspects of routine histological and immunohistochemical procedures. The Core is equipped with cutting-edge technologies to process, embed and perform histological and histomorphometric evaluation.
The core has necessary facilities to conduct histochemical and immunohistochemical analysis, in situ hybridization, and imaging/microscopy of tissues and cells. This facility is staffed by a full-time certified histotech (Ms. Renee Till) who oversees the daily technical activities including the maintenance of equipment and the standardization of techniques. The facility also helps investigators in developing new procedures and special stains as needed.
The facility also has several fluorescence and light microscopes including a Zeiss Axiovert and an Olympus upright BX50. Accompanying software with histomorphometry, density scanning and 3-dimensional reconstruction and rendering capabilities are also available. Stereo/ dissecting microscopes are available for micromanipulation, microsurgery and other purposes. The facility has an automated Sakura Tissue Tek Vacuum Infiltration Processor for processing of tissues and a Tissue Tek Embedding platform. The facility also has Leica RM2135 microtome for cutting paraffin or acrylic embedded blocks. For performing analyses on frozen sections the laboratory utilizes a Microm HM550 cryostat. The facility has standardized protocols for histochemical, immunohistochemical and in situ hybridization staining that can either be done manually or performed using an automated Dako autostainer. The Histology facility also has general laboratory equipment necessary for effective functioning including slide warmers, ovens and incubators dedicated for histological procedures.
Renee Fox, Research Assistant