Metabolomics is the study of small molecules, collectively called metabolites, within biofluids, tissues and organs. These molecules and their interaction within a biological system is called the metabolome, and changes in the metabolome often track health, changes in diet, and metabolism. The ACNC Metabolomics core assists ACNC investigators and collaborators in the analysis of metabolite profiles in a variety of human and animal samples, including plasma, serum, tissues, urine, and fecal/cecal contents using both untargeted and target liquid chromatography mass spectrometry (LC-MS) methods.
The Core employs a full-time chemist responsible for the operation and maintenance of two LC/MS systems: a Thermo Q-Exactive hybrid quadrupole-orbitrap mass spectrometer coupled with an Ultimate 3000 RSLCnano UHPLC, and a SCIEX 4000 QTRAP® mass spectrometer coupled to an Ultimate 3000® SD HPLC. Processing and analysis software includes Analyst 1.7, Xcalibur 4.0, Tracefinder 3.3., Compound Discoverer 2.0, and M/Z Vault; the latter contains our in-house, curated MS2-spectral database. The Core also runs standard clinical chemistry analyses in high throughput using a RX Daytona Analytical Analyzer (Randox, Kearneysville, WV). In addition to the routine LC-MS assays, our facility continues to expand our repertoire of methods by working closely with the ACNC investigators to develop and standardize new assays to analyze metabolites.
Histology and Bioimaging Core Staff
Kelly Mercer, Ph.D., Core Director
Lindsay Pack, MS, Research Associate