Histology & Bioimaging Core
The ACNC Histology and Bioimaging Core assists ACNC investigators with all aspects of routine histological and immunohistochemical procedures. The Core is equipped with state-of-the-art technologies to process, embed and perform routine histological and histomorphometric evaluation.
The core has necessary facilities to conduct histochemical, immunohistochemical, in situ hybridization, and imaging/microscopy analysis of tissues and cells. A full-time certified histotech oversees the daily activities including the maintenance of equipment, project management and the development and standardization of new techniques. The facility also helps investigators in developing new procedures and special stains as needed.
The facility also has several fluorescence and light microscopes including a Nikon Eclipse Ti2 inverted microscope, a Zeiss Axiovert and an Olympus upright BX50. The core has ability to conduct brightfield and multi-color fluorescence imaging in both live cells, and processed cells and tissues. Equipment to conduct automated image acquisition and analysis are available including high-throughput acquisition in 96-well formats. Accompanying software with for image analysis and histomorphometry, density scanning and 3-dimensional reconstruction and rendering capabilities are also available. Several stereomicroscopes with brightfield and epi- fluorescence capabilities are present. The facility is equipped with an automated Sakura Tissue Tek Vacuum Infiltration Processor for processing of tissues, a Tissue Tek Embedding platform, and a Leica RM2135 microtome for cutting paraffin embedded blocks. For performing analyses on frozen sections, the laboratory utilizes a Microm HM550 cryostat. A Dako autostainer is also available.
Histology and Bioimaging Core Staff
Reneé Fox, HT(ASCP), Research Assistant